AI TriPeptide 29
45
Human Collagen Peptide Sequences
940
Sequence Patterns to Identify Optimal Candidates
What is Tripeptide-29?
Tripeptide-29 is a biomimetic tripeptide composed of glycine, proline and hydroxyproline (Gly-Pro-Hyp), an amino-acid sequence associated with the structure of collagen. Used in advanced cosmetic formulations as a skin-conditioning peptide, Tripeptide-29 is studied for its potential to support collagen synthesis, extracellular matrix integrity and the visible signs of skin aging. La Exomé uses AI-assisted peptide screening alongside laboratory and human clinical evaluation to investigate its cosmetic performance.
A Comprehensive Approach to DNA Protection, Collagen Stimulation & Brightening
Clinically validated across multiple biological pathways, this advanced ingredient delivers comprehensive skin rejuvenation by acting at both the cellular
and structural levels. It helps protect DNA from UV and oxidative damage, while modulating key signaling pathways to reduce inflammation and cellular
stress, creating an optimal environment for healthy skin function. At the same time, it activates longevity-related proteins and significantly enhances the
expression of multiple collagen types (I, III, IV, VII), supporting improved firmness, elasticity, and skin integrity. Complementing these effects, it also reduces
transepidermal water loss and inhibits melanin production, promoting long-lasting hydration and a more even, radiant complexion.
The result is visibly smoother, firmer, and brighter skin—empowered from within for a refined, youthful appearance.
Tripeptide-29 Increased Type I Collagen Production by 148% in In Vitro Testing
In vitro testing using human skin cells demonstrated that Tripeptide-29 significantly increased Type I collagen production after 24 hours of exposure.
At a concentration of 0.01% (100 ppm), Tripeptide-29 produced an up to 148% increase in Type I collagen production under the study conditions.
Key Study Result
- Ingredient tested: Tripeptide-29
- Concentration: 0.01% (100 ppm)
- Test model: Human skin cells
- Exposure period: 24 hours
- Measured marker: Type I collagen production
- Result: Up to +148% increase in Type I collagen production
- Comparison groups: Untreated control, conventional collagen booster and TGF-β positive control
Why Type I Collagen Matters for Skin
Type I collagen is one of the major structural proteins found in the skin’s extracellular matrix. It contributes to the skin’s firmness, strength and structural support.
Collagen levels naturally decline with age and can also be affected by environmental stressors. Ingredients that support collagen production are therefore of particular interest in advanced anti-aging skincare research.
How Does Tripeptide-29 Support Collagen?
Tripeptide-29 is a biomimetic peptide composed of the amino-acid sequence Gly-Pro-Hyp (glycine-proline-hydroxyproline), a sequence associated with collagen structure.
La Exomé’s research evaluates Tripeptide-29 for its potential to support pathways associated with collagen synthesis and extracellular matrix maintenance, helping promote a firmer and more resilient-looking complexion.
Does Tripeptide-29 increase collagen?
Laboratory testing using human skin cells found that 0.01% (100 ppm) Tripeptide-29 increased Type I collagen production by up to 148% after 24 hours under the study conditions. This result was obtained in vitro and should be distinguished from results obtained in human clinical testing.
Tripeptide-29 → 0.01% / 100 ppm → human skin cells → 24 hours → Type I collagen → +148% → in vitro.
Advanced Elasticity Restoration
By strengthening the skin’s elastic network, it helps improve resilience, reduce the appearance of sagging, and restore a firmer, more lifted look. The result: skin that feels more supple, resilient, and visibly youthful with enhanced bounce and structural support
Collagen Preservation & Anti-Aging Protection
In vitro testing demonstrated that La Exomé Tripeptide-29 reduced the activity of key collagen-degrading enzymes, MMP-1 and MMP-3, by approximately 50% compared with the control.
MMPs (matrix metalloproteinases) are enzymes involved in the breakdown of the skin’s extracellular matrix. MMP-1 is associated with collagen degradation, while MMP-3 contributes to the breakdown of structural components that help maintain skin firmness and elasticity.
In vitro testing demonstrated that La Exomé Tripeptide-29 reduced the activity of key collagen-degrading enzymes, MMP-1 and MMP-3, by approximately 50% compared with the control.
MMPs (matrix metalloproteinases) are enzymes involved in the breakdown of the skin’s extracellular matrix. MMP-1 is associated with collagen degradation, while MMP-3 contributes to the breakdown of structural components that help maintain skin firmness and elasticity.
By helping reduce MMP-1 and MMP-3 activity, Tripeptide-29 may support the preservation of collagen and the skin’s structural matrix, helping maintain a firmer, smoother and more resilient-looking complexion while addressing visible signs of skin aging such as wrinkles and loss of elasticity.
Key In Vitro Findings
MMP-1: approximately 50% reduction versus control
MMP-3: approximately 50% reduction versus control
Research focus: collagen preservation and extracellular matrix protection
Ingredient studied: Tripeptide-29
Test type: In vitro laboratory study
Results are based on in vitro laboratory testing and do not necessarily represent results achieved on human skin during normal cosmetic use.
Tripeptide-29 → MMP-1 ↓50% → MMP-3 ↓50% → reduced collagen degradation → collagen preservation → skin firmness and elasticity.
Clinically Proven Skin Firming Performance
Human Clinical Study: Tripeptide-29 Improves Skin Firmness
A controlled in vivo human clinical study evaluated the effect of La Exomé Tripeptide-29 on skin firmness in 32 middle-aged women with an average age of 46.4 ± 1.2 years.
Participants applied a formulation containing 0.1% La Exomé Tripeptide-29 once daily for 28 days. Skin firmness was objectively assessed using the standardized F4 skin firmness parameter, where a higher F4 value indicates firmer skin.
Measurable improvements were observed as early as Day 14, with skin firmness continuing to improve through Day 28. At the end of the four-week study, the Tripeptide-29 formulation produced a statistically significant increase in skin firmness of up to +13.5% compared with the base formulation.
These human clinical findings demonstrate that daily use of a formulation containing 0.1% Tripeptide-29 for four weeks can measurably improve skin firmness, supporting its use as an advanced anti-aging peptide for maintaining a firmer, tighter and more resilient-looking complexion.
Key Clinical Study Findings
Study type: Controlled in vivo human clinical study
Participants: 32 middle-aged women
Average age: 46.4 ± 1.2 years
Tripeptide-29 concentration: 0.1%
Application: Once daily
Study duration: 28 days
Measurement: F4 skin firmness parameter
Day 14: Measurable improvement in skin firmness
Day 28: Up to +13.5% improvement in skin firmness
Result: Statistically significant improvement versus the base formulation
What Does This Mean for Skin?
Skin firmness naturally declines with age as collagen, elastin and other components of the extracellular matrix become reduced or structurally altered. By supporting pathways associated with skin structure, Tripeptide-29 may help improve the appearance of firmness, elasticity and overall skin resilience with continued use.
Results are based on a 28-day clinical study using a formulation containing 0.1% Tripeptide-29. Individual results may vary.
What Does Improved Skin Elasticity Mean?
Skin elasticity refers to the skin’s ability to stretch, recover and return to its original form. As skin ages, changes in collagen and elastin can contribute to reduced bounce, increased laxity and the appearance of fine lines and sagging.
By supporting skin elasticity, Tripeptide-29 may help maintain a firmer, more flexible and resilient-looking complexion, making it a valuable peptide for advanced anti-aging skincare formulations.
Does Tripeptide-29 Improve Skin Elasticity?
In a 28-day human clinical study involving 32 women, daily application of a formulation containing 0.1% La Exomé Tripeptide-29 resulted in an improvement in skin elasticity of up to 30.6%, with measurable changes observed from Day 14.
Results are based on a 28-day human clinical study using a formulation containing 0.1% Tripeptide-29. Individual results may vary.
Human Clinical Study: Tripeptide-29 Reduces Wrinkles by Up to 9.8%
A human clinical study evaluated the effects of a serum containing 0.1% La Exomé Tripeptide-29 on skin firmness and the appearance of wrinkles in middle-aged women aged 45–59 years.
Participants applied the Tripeptide-29 serum once daily for 28 days. After four weeks of use, skin analysis demonstrated a 15.6% improvement in skin firmness and a 9.8% reduction in wrinkles under the study conditions.
The results indicate that consistent use of a formulation containing 0.1% Tripeptide-29 can help improve visible signs of skin aging, including loss of firmness and the appearance of wrinkles, supporting a smoother, firmer and more youthful-looking complexion.
Tripeptide-29 Increases Fibronectin Production by 96%
In vitro testing using human skin cells demonstrated that La Exomé Tripeptide-29 increased fibronectin production by up to 96% compared with the control under the study conditions.
Fibronectin is a key extracellular matrix protein involved in supporting the organization, adhesion and structural stability of the skin. It plays an important role in maintaining the environment that supports collagen and elastin networks, which are essential for skin firmness, elasticity and resilience.
By increasing fibronectin production, Tripeptide-29 may help reinforce the skin’s extracellular matrix and support its underlying structural architecture, contributing to a firmer, smoother and more resilient-looking complexion.
Why Is Fibronectin Important for Skin Firmness?
Fibronectin is an extracellular matrix glycoprotein that helps organize and support the structural network surrounding skin cells. This matrix provides an important framework for proteins such as collagen and elastin, which contribute to the skin’s strength, firmness and elasticity.
Supporting fibronectin production may therefore help maintain a stronger and more resilient extracellular matrix, an important consideration in advanced anti-aging skincare research.
Does Tripeptide-29 Support Skin Firmness?
Laboratory testing using human skin cells showed that La Exomé Tripeptide-29 increased fibronectin production by up to 96% compared with the control. As fibronectin plays an important role in extracellular matrix organization and collagen support, these findings suggest that Tripeptide-29 may help support the structural foundations associated with firmer and more resilient-looking skin.
Results are based on in vitro laboratory testing. In vitro findings do not necessarily represent results achieved on human skin during normal cosmetic use.
Clinical Trial: Boost Fibronectin Production
Clinically Proven Cellular Inflammation Reduction
Clinically Proven Cellular Inflammation Reduction
Clinical Trial: Improves Skin Hydration
Clinical Trial: Improves Skin Hydration
Clinical Trial: Reduce Redness
Clinical Trial: Reduce Redness
Skin Penetration Test
Skin Penetration Test
